Written by
in
2. Which of the following heavy/radioisotopes is not suitable for DNA labelling based studies?
(a) 3H(b) 32p(c) 15N (d) 35S
3. A molecule to act as genetic material has the following properties (i) Should be able to replicate (ii) Structurally more stable (iii) Should be more reactive and labile (iv) Should provide scope for slow changes Choose the correct option.(a) (i), (ii) and (iii) (b) (iii) only (c) (iii) and (iv) (d) (i), (ii) and (iv)
4. In Hershey and Chase experiments, radioactive 32P was used to culture bacteriophages. It resulted in radioactive(a) viral proteins (b) viral DNA (c) plasma membrane of bacteria (d) bacterial capsule.
5. Transformation experiments in bacteria were first performed by(a) MacLeod(b) Griffith (c) Pasteur (d) Meselson and Stahl.
6. Griffith’s effect is called(a) transduction (b) transformation (c) translocation (d) all of these.
7. DNA replication is semiconservative as(a) only non-parent strand acts as template (b) both strands of new molecule are synthesised de novo (c) one of the strand in each new molecule is parental and the other is new (d) daughter strands are dispersive.
8. In DNA replication, the leading strand is the one which replicates in(a) 5’3′ direction continuously (b) 3’5′ direction continuously (c) 5’3′ direction discontinuously (d) 3’5′ direction discontinuously
9. Gel electrophoresis is a(a) technique of separation of charged molecules under the influence of magnetic field (b) technique of incorporation of DNA molecules into the cell through transient pores made due to electrical impulses (c) technique of separation of DNA fragments through the pores of agarose gel under the influence of electric field (d) none of these.
10. In pBR322, tetracycline resistance gene (tet”) has recognition site for which of the following restriction endonuclease?(a) HindIII (b) BamHI (c) EcoRI (d) Pstl
11. Back bone in structure of DNA molecule is made up of(a) pentose sugar and phosphate (b) hexose sugar and phosphate (c) purine and pyrimidine (d) triose sugar and phosphate
12. On an average, how many nitrogenous bases are present in one turn of DNA?(a) Four (b) Five (c) Ten (d) Eighteen
13. Central dogma of molecular biology was modified with the discovery of(a) ligase (b) RNA polymerase (c) DNA polymerase (d) reverse transcriptase
14. Agarose extracted from sea weeds finds use in(a) Chromatography (b) tissue culture (c) PCR (d) reverse transcriptase
15. PBR322 has two antibiotic resistance genes. These are against(a) chloramphenicol and tetracycline (b) kanamycin and chloramphenicol (c) ampicillin and tetracycline (d) ampicillin and kanamycin.
16. The first step in recombinant DNA technology is(a) isolation of DNA (b) insertion of DNA (c) joining of sticky ends of DNA with its complementary DNA fragment (d) multiplication of DNA.
17. During transcription, RNA polymerase binds to DNA site(a) regulator (b) promoter (c) enhancer (d) receptor
18. In eukaryotes, RNA polymersa III catalyses the synthesis(a)18 S rRNA(b) tRNA (c)mRNA (d)5.8 rRNA
19. Intron is part of RNA which(a) codes for protein synthesis (b) helps in joining pieces of DNA (c) does not code for protein synthesis (d) initiates transcription
20. tRNA attaches to mRNA and amino acid respectively at(a) anticodon loop and -CCA end (b) -CCA end and anticodon loop (c) anticodon loop and DHU loop (d) DHU loop and -CCA end.